具有优化金属增强荧光效果的一维核壳结构纳米材料的制备及其在生物医学应用研究

The core-shell structure nanocomposites exhibit special physical and chemical properties due to their composition, size and morphology. If these factors are controlled effectively, the physical and chemical properties can be greatly improved. Polydiacetylene (PDA) polymers have been widely used as a variety of sensing materials due to their unique fluorescence recognition. However, due to the low fluorescence quantum yield, it hinders further application of PDA. In this project, core-shell nanomaterials are prepared with gold nanorod (AuNR) as core and PDA as shell. By adjusting the distance between PDA and AuNR, and the aspect ratio of AuNR, simultaneously, PDA can achieve the best effect of metal enhanced fluorescence (MEF). In addition, the interaction between AuNR@PDA and CA125 antigen proteins is studied. Based on this, fluorescent diagnosis, imaging, and photo-thermal therapy of ovarian cancer SKOV-3 cells are studied. It will helpful for us to expand the potential application of the core-shell nanomaterials in the field of biomedical research.

核壳结构纳米复合材料因其组成、尺寸及形貌的不同体现出特殊的物理和化学性质。若有效地调控这些因素，能使一些物化性质得到极大的提高。 聚丁二炔（PDA）聚合物因其独特的荧光识别功能，广泛应用为各种传感材料。但由于PDA聚合物的荧光量子产率比较低，阻碍了它的进一步应用。本项目拟制备以金纳米棒 (AuNR) 为内核， PDA聚合物为外壳的核壳结构纳米材料，首次通过同时调控PDA与AuNR之间的距离及AuNR的长径比，使PDA达到最优金属增强荧光（MEF）效果。结合AuNR 本身的光学特性，研究AuNR@PDA纳米材料与CA125抗原蛋白之间的相互作用，并以此为基础对卵巢癌SKOV-3细胞进行荧光靶向诊断、成像以及光热治疗研究，拓展此类材料在生物医学研究领域的潜在应用价值。

本项目基于贵金属纳米粒子的代表物质，金纳米棒（gold nanorod, GNR）为主体，制备了几种核壳型纳米材料，分别应用于生物成像、热消融以及光催化等领域。具体成果如下：.1. 通过调控GNR的长径比，以及二氧化硅（SiO2）夹板层的厚度，是两种外壳层物质，聚丁二炔（Polydiacetylene, PDA）和Cy5.5荧光团的发光强度分别得到了极大的优化。此外，利用优化的GNR@SiO2-Cy5.5纳米材料对卵巢癌细胞进行了成像及热消融治疗。本纳米材料展现了清晰的成像效果和热消融治疗效果。.2. 制备了一种以不同长径比的GNR为内核，无孔（pinhole free, pf）超薄SiO2为外壳的核壳型纳米材料。利用内核GNR的等离子体效应，将此纳米材料应用于非接触型光催化研究，并在对硝基苯酚的还原反应中证明了较好的催化效果。