丹酚酸 B 干预口腔白斑恶变的线粒体蛋白质组学研究

Oral leukoplakia is one of the important sources of oral squamous cell carcinoma. It is very important that taking effective means to block the malignant transformation of oral leukoplakia for oral cancer prevention. Studies showed that precancerous lesions have already had abnormal cellular energy metabolism performance.More and more evidence demonstrated that the energy metabolism of tumor cells is closely related to tumor-generated signal transduction pathway. Our preliminary studies showed that elevated glutaminolysis and glycolysis, the Krebs cycle disorders, abnormal branched-chain amino acid metabolism have been seen in oral leukoplakia malignant transformation .On the basis of previous studies, this project will combine mitochondrial proteomics technology with animal experiments and cytobiology, and will use two dimension difference gel electrophoresis(2D-DIGE)and Matrix-Assisted Laser Desorption/ Ionization Time of Flight Mass Spectrometry(MALDI-TOF-MS) technology to screen different protein after salvianolic acid B intervening the malignant transformation of leukoplakia.Then We will use bioinformation technology to analyse energy metabolism pathways.

口腔白斑是口腔鳞癌的重要来源之一，采取有效手段阻断其恶性转化对口腔癌的预防具有重要意义。研究表明癌前病变阶段就有细胞能量代谢异常的表现，越来越多的证据证明肿瘤细胞能量代谢与肿瘤生成的信号转导通路密切相关。申请人前期研究发现在口腔白斑恶变的过程中有糖酵解增强、三羧酸循环紊乱，谷氨酰胺代谢增强、支链氨基酸代谢异常等能量代谢异常表现。本项目在既往研究工作的基础上，将线粒体蛋白质组学技术与动物实验、细胞分子生物学相结合，采用双向荧光差异凝胶电泳（2D-DIGE）结合基质辅助激光解析飞行时间质谱(MALDI-TOF-MS)技术对丹酚酸B干预金地鼠颊囊黏膜白斑癌变过程的差异表达蛋白质进行筛选鉴定；应用生物信息技术分析影响能量代谢的相关通路进行功能研究。